Application

See technical bulletin for detailed protocols

Features and Benefits

Zinc finger nucleases (ZFN) mediated Knockout of ABCG2 (BCRP) gene. The frame-shift mutation of ABCG2 gene was confirmed by fragment length analysis and DNA sequencing. Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.

HepaRGcells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG BCRP Knockout (KO) allows investigations of drug-transporter interactions involving BCRP in the liver.

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG BCRP knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Legal Information

HepaRG is a trademark of BioPredic International company

Exhibit 1: ADME/Tox cell lines license Exhibit 2: HepaRG limited use license

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.